Table 2. Nitrotryptophan-containing trypsin-digested peptide sequnces identified from hippocampus.

Boldface W indicates nitrated tryptophan residue. All peptides cleared P<0.05 of the ion scores by the Mascot search. Supplementary Figures S4—S16 are available online at http://www.bioscirep.org/bsr/032/bsr0320521add.htm.

Protein	Peptide sequence	MS/MS spectrum
PDCEZ, mitochondrial	K475.VPEANSSW483MDTVIR.Q490	Supplementary Figure S4
	R606.VVDGAVGAQW616LAEFKK.Y623	Supplementary Figure S5
Tubulin β-2A, -2B, -2C and -5 chains	R77.SGPFGQIFRPDNFVFGQSGAGNNW101AK.G104*	Supplementary Figure S6
α-Enolase	K358.LAQSNGW365GVMVSHR.S373	Supplementary Figure S7
Glutamine synthetase	K25.IQLMYIW32VDGTGEGLR.C42	Supplementary Figure S8
γ-Enolase	K358.LAQENGW365GVMVSHR.S373	Supplementary Figure S9
Actin, cytoplasmic 2†	K68.YPIEHGIVTNW79DDMEK.I85	Supplementary Figure S10
Fructose-bisphosphate aldolase C	R304.ALQASALSAW314R.G315	Figure 2
Fructose-bisphosphate aldolase A	R304.ALQASALKAW314GGK K318	Supplementary Figure S11
GAPDH	K84. W85GDAGAEYVVESTGVFTTMEK.A106	Supplementary Figures S12
	K307.LISW311YDNEYGYSNR.V322	Supplementary Figures S13
Electron transfer flavoprotein subunit α, mitochondrial	K187.APSSSSAGISEW199LDQK.L204	Supplementary Figure S14
Phosphoglycerate kinase 1‡	K382. W383NTEDKVSHVSTGGGASLELLEGK.V407	Supplementary Figure S15
Creatine kinase U-type, mitochondrial	K257.SFLIW262VNEEDHTR. V271	Supplementary Figure S16

*This peptide sequence is identical among four isoforms of tubulin β identified in the present study, and tubulin β was counted as one protein.

^†Methylation of histidine residue (+14 Da) is added for the identification of nitrated tryptophan residue.

^‡Formylation of lysine residue (+28 Da) is added for the identification of nitrated tryptophan residue.