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. 2012 Sep 14;32(6):521–530. doi: 10.1042/BSR20120032

Table 3. NO2Trp-containing trypsin-digested peptide sequences identified from cerebellum.

Boldface W indicates nitrated tryptophan residue. All peptides cleared P<0.05 of the individual ion scores by the Mascot search. Supplementary Figures S17–S23 are available online at http://www.bioscirep.org/bsr/032/bsr0320521add.htm.

Protein Peptide sequence MS/MS spectrum
Tubulin β-2A, -2B, -2C and -5 chains R77.SGPFGQIFRPDNFVFGQSGAGNN101WAK. G104* Supplementary Figure S17
α-Enolase K358.LAQSNGW365GVMVSHR.S373 Supplementary Figure S18
γ-Enolase K358.LAQENGW365GVMVSHR.S373 Supplementary Figure S19
Actin, cytoplasmic 2 K68.YPIEHGIVTNW79DDMEK.I85 Supplementary Figure S20
Fructose-bisphosphate aldolase C R304.ALQASALSAW314R.G315 Supplementary Figure S21
GAPDH K307.LISW311YDNEYGYSNR.V322 Supplementary Figure S22
Voltage-dependent anion-selective channel protein 1 K61.YRW64TEYGLTFTEK.W75 Supplementary Figure S23

*This peptide sequence is identical among four isoforms of tubulin β identified in the present study, and tubulin β was counted as one protein.

Methylation of His (+14 Da) is added for the identification of nitrated tryptophan residue.