Table 3. NO2Trp-containing trypsin-digested peptide sequences identified from cerebellum.
Protein | Peptide sequence | MS/MS spectrum |
---|---|---|
Tubulin β-2A, -2B, -2C and -5 chains | R77.SGPFGQIFRPDNFVFGQSGAGNN101WAK. G104* | Supplementary Figure S17 |
α-Enolase | K358.LAQSNGW365GVMVSHR.S373 | Supplementary Figure S18 |
γ-Enolase | K358.LAQENGW365GVMVSHR.S373 | Supplementary Figure S19 |
Actin, cytoplasmic 2† | K68.YPIEHGIVTNW79DDMEK.I85 | Supplementary Figure S20 |
Fructose-bisphosphate aldolase C | R304.ALQASALSAW314R.G315 | Supplementary Figure S21 |
GAPDH | K307.LISW311YDNEYGYSNR.V322 | Supplementary Figure S22 |
Voltage-dependent anion-selective channel protein 1 | K61.YRW64TEYGLTFTEK.W75 | Supplementary Figure S23 |
*This peptide sequence is identical among four isoforms of tubulin β identified in the present study, and tubulin β was counted as one protein.
†Methylation of His (+14 Da) is added for the identification of nitrated tryptophan residue.