Figure A1.

Multiple sequence alignment of NrfA from B. azotoformans LMG 9581^T, B. bataviensis LMG 21833^T, and other bacteria. Heme-binding motifs are highlighted in red (white lettering), the lysine at the catalytic heme is highlighted in green, active site residues are printed in blue, distal heme-ligating histidines for hemes 2–6 are printed in red [according to Einsle et al. (2000)]. Bs, Bacillus selenitireducens MLS10 (Bsel_1305); Dv, Desulfovibrio vulgaris DP4 (PDB 2J7A); Dd, Desulfovibrio desulfuricans (PDB 10AH_A). PDB, protein database accession number.