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Published in final edited form as: Drug Dev Res. 1996 Sep 1;39(1):36–46. doi: 10.1002/(sici)1098-2299(19960901)39:1<36::aid-ddr5>3.0.co;2-l

Concentration-dependent stimulation of phosphoinositide breakdown by adenosine analogs in RBL-2H3 mast cells. A: [³H]IP₁. B: [³H]IP₂. Accumulation of [³H]inositol phosphates was measured as described in Methods in the absence or presence of adenosine analogs. Analogs were as follows: NECA, 2-chloroadenosine (2CIADO); 2Cl-IBMECA; N⁶-3-iodobenzyl-MECA (IBMECA); N⁶-benzyladenosine (BzADO); N⁶-R-phenylisopropyladenosine (R-PIA); N⁶-cyclohexylNECA (CHNECA); CGS 21680; APNEA; N⁶-cyclohexyladenosine (CHA); adenosine (ADO); 8-bromoadenosine (8-BrADO); 1,3-dibutylxanthine 7-riboside-5′-N-methylcarboxamide (DBXRM). The analogs were tested in the concentration range of 0.3 to 30 µM (open bars control; dotted bars 0.3 µM; hatched bar 1 µM; striped bar 3 µM; heavy dotted bar 10 µM; solid bar 30 µM). Incubations were for 1 min with cells pretreated with the DNP-specific IGE and then challenged with the antigen DNP-HSA. Basal accumulations of [³H]IP₁ and [³H]IP₂ for the 1-min incubations were 258 ± 21 and 143 ± 10 cpm/10,000 cpm membrane lipids, respectively. Values are means ± s.e.m. (n = 3). *p <0.05.

Concentration-dependent stimulation of phosphoinositide breakdown by adenosine analogs in RBL-2H3 mast cells. A: [³H]IP₁. B: [³H]IP₂. Accumulation of [³H]inositol phosphates was measured as described in Methods in the absence or presence of adenosine analogs. Analogs were as follows: NECA, 2-chloroadenosine (2CIADO); 2Cl-IBMECA; N⁶-3-iodobenzyl-MECA (IBMECA); N⁶-benzyladenosine (BzADO); N⁶-R-phenylisopropyladenosine (R-PIA); N⁶-cyclohexylNECA (CHNECA); CGS 21680; APNEA; N⁶-cyclohexyladenosine (CHA); adenosine (ADO); 8-bromoadenosine (8-BrADO); 1,3-dibutylxanthine 7-riboside-5′-N-methylcarboxamide (DBXRM). The analogs were tested in the concentration range of 0.3 to 30 µM (open bars control; dotted bars 0.3 µM; hatched bar 1 µM; striped bar 3 µM; heavy dotted bar 10 µM; solid bar 30 µM). Incubations were for 1 min with cells pretreated with the DNP-specific IGE and then challenged with the antigen DNP-HSA. Basal accumulations of [³H]IP₁ and [³H]IP₂ for the 1-min incubations were 258 ± 21 and 143 ± 10 cpm/10,000 cpm membrane lipids, respectively. Values are means ± s.e.m. (n = 3). *p <0.05.