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Immunosuppresive activity of hMSC conditioned media. hMSCs were cultured for 24 hours in ITS+ -supplemented RPMI with IL-1β (5 pg/ml), TNF-α (25 ng/ml), or both IL-1β and TNF-α; unstimulated cultures were maintained in ITS+-supplemented RPMI. Control samples were prepared incubating the media with and without the cytokines in the absence of hMSCs. The immunosuppressive activity of the conditioned media was assessed in elispots.
