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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Biochemistry. 2012 Sep 19;51(39):7733–7739. doi: 10.1021/bi3009054

Figure 4.

Figure 4

A) A schematic representation of the isotopic scrambling process that would be observed if positional isotope exchange (PIX) occurred in the CymD reaction. B) 31P NMR spectra showing the PIX process during the reaction of 4-fluorotryptophan and [1-18O]-DMAPP with CymD. Only the signals for the α-phosphorus of DMAPP are shown. The upper spectrum shows DMAPP before the addition of enzyme. The lower spectrum shows DMAPP after 59% had been consumed. Darkened atoms represent 18O isotopic labels.