Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Enzyme kinetic plots of initial velocity/[E] vs. substrate concentration demonstrating a solvent kinetic isotope effect. Tryptophan is the variable substrate and saturating DMAPP (20 μM) was employed. Triangles represent data obtained in H2O and squares represent data obtained in > 95% D2O. Data was fit to the Michaelis-Menten equation (dashed line = H2O and solid line = D2O).
