Table 2.
The expected distributions for the number of steps calculated using the binomial distribution for different scenarios of channel stoichiometry
| Steps |
|||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| β-VFP | |||||
| Data | 36% | 62% | 2% | 0% | 0% |
| Expected for 2 β-subunits and p = 78% | 36% | 64% | 0% | 0% | 0% |
| Expected for 3 β-subunits and p = 78% | 11% | 41% | 48% | 0% | 0% |
| α-VFP | |||||
| Data | 18% | 30% | 43% | 6% | 3% |
| Expected for 3 α-subunits, p = 78%, homomers = 15% | 9% | 36% | 44% | 6% | 5% |
| Expected for 2 α-subunits, p = 78%, homomers = 15% | 31% | 55% | 3% | 6% | 5% |
The probability for VFP to be fluorescent was set as 78%. In the case of α1-VFP:β channels, the percentage of homomers was additionally set as 15%. Comparison of data with different scenarios of heteromeric GlyR stoichiometry.