Table 1.
m/z, detection limit and migration reproducibility of BAMP and HAMP derivatives of carboxylic acids.
| Peak | Carboxylic acid | BAMP | HAMP | Migration time RSD (%, n=5)b | ||||
|---|---|---|---|---|---|---|---|---|
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| m/z | Detection limita | m/z | Detection limit | |||||
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| Conc. (μM) | Mass (pg) | Conc. (μM) | Mass (pg) | |||||
| 1 | Oxaloacetic acid, di- | 213.37 (+2) | 20.0 | 26.0 | 241.38 (+2) | 5.0 | 6.0 | 1.20 |
| 2 | Oxalic acid, di- | 192.35 (+2) | 20.0 | 18.0 | 220.40 (+2) | 5.0 | 4.0 | 1.11 |
| 3 | Malonic acid, di- | 199.33 (+2) | 10.0 | 10.0 | 227.36 (+2) | 2.0 | 2.0 | 0.92 |
| 4 | Fumaric acid, di- | 205.37 (+2) | 5.0 | 5.0 | 233.35 (+2) | 1.0 | 1.0 | 1.16 |
| 5 | Succinic acid di- | 206.36 (+2) | 2.0 | 2.0 | 234.37 (+2) | 0.5 | 0.6 | 0.99 |
| 6 | Maleic acid, di- | 205.37 (+2) | 5.0 | 5.0 | 233.35 (+2) | 1.0 | 1.0 | 1.29 |
| 7 | Malic acid, di- | 214.37 (+2) | 2.0 | 2.0 | 242.39 (+2) | 0.5 | 0.6 | 1.18 |
| 7 | Citric acid, di-, -H2O | 234.33 (+2) | 5.0 | 9.0 | 262.36 (+2) | 1.0 | 2.0 | 1.18 |
| 7 | ketoglutaric acid, di- | 220.38 (+2) | 10.0 | 14.0 | 248.38 (+2) | 2.0 | 3.0 | 1.18 |
| 8 | isocitric acid, di-, -H2O | 234.33 (+2) | 10.0 | 19.0 | 262.36 (+2) | 2.0 | 4.0 | 1.23 |
| 9 | Tartaric acid, di- | 222.36 (+2) | 5.0 | 7.0 | 250.37 (+2) | 1.0 | 1.0 | 1.26 |
| 10 | Adipic acid, di- | 220.57 (+2) | 2.0 | 2.0 | 248.38 (+2) | 0.5 | 0.7 | 1.32 |
| 11 | Citric acid, di- | 243.33 (+2) | 5.0 | 9.0 | 271.33 (+2) | 1.0 | 2.0 | 1.01 |
| 12 | isocitric acid, di- | 243.33 (+2) | 5.0 | 9.0 | 271.03 (+2) | 1.0 | 2.0 | 1.00 |
| 12 | Formic acid | 193.33 (+1) | 5.0 | 2.0 | 221.32 (+1) | 1.0 | 0.5 | 1.00 |
| 13 | Acetic acid | 207.34 (+1) | 10.0 | 6.0 | 235.33 (+1) | 5.0 | 3.0 | 1.06 |
| 14 | Propionic acid | 221.37 (+1) | 2.0 | 1.0 | 249.14 (+1) | 1.0 | 0.7 | 1.08 |
| 15 | Pyruvic acid | 235.37 (+1) | 10.0 | 8.0 | 263.37 (+1) | 2.0 | 1.0 | 1.14 |
| 15 | Lactic acid | 237.34 (+1) | 10.0 | 9.0 | 265.37 (+1) | 2.0 | 2.0 | 1.14 |
| 16 | Butyric + isobutyric acid c | 235.32 (+1) | 2.0 | 1.0 | 263.37 (+1) | 1.0 | 0.8 | 1.20 |
| 17 | Benzonic acid | 269.33 (+1) | 2.0 | 2.0 | 297.32 (+1) | 0.5 | 0.6 | 1.28 |
| 18 | Shikimic acid | 321.36 (+1) | 10.0 | 17.0 | 349.34 (+1) | 2.0 | 3.0 | 1.24 |
| 19 | Quinic acid | 339.35 (+1) | 100.0 | 192.0 | 367.36 (+1) | 20.0 | 38.0 | 1.27 |
a
Mean of three injections of five different levels in the concentration range of 100-1mM, injection volume 10 nl.
b
Consecutive injections (n = 5) of standard mixture at the concentration of 100mM.
c
Not separated by CE/MS.