Table 1.
Peptide charge and length distribution for Lys-C digestion ETD dataset and corresponding clusters.
| All (11954 peptides) |
Cluster 1 (3522 peptides) |
Cluster 2 (4737 peptides) |
Cluster 3 (3695 peptides) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Charge | 2 | 14% | 36% | 3% | 8% | ||||
| 3 | 45% | 38% | 60% | 33% | |||||
| 4 | 29% | 21% | 29% | 36% | |||||
| 5 and more | 12% | 5% | 9% | 22% | |||||
| Average charge | 3.4 | 3.0 | 3.5 | 3.7 | |||||
| Average length | 17 | 14 | 14 | 21 | |||||
| Sequence with internal Lys |
28% | 13% | 31% | 38% | |||||
| Sequence with internal Arg |
63% | 54% | 66% | 66% | |||||
| Fragmentation patterns |
N/A | Very strong X-K cleavage (Cnā1 ion) |
Moderate cleavage for selected residue pairs |
No cleavage preference |
|||||