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Peptide charge and length distribution for Glu-C digestion ETD dataset and corresponding clusters.
| All (12042 peptides) |
Cluster 1 (5340 peptides) |
Cluster 2 (6702 peptides) |
|||||
|---|---|---|---|---|---|---|---|
| Charge | 2 | 1% | 0% | 1% | |||
| 3 | 45% | 32% | 56% | ||||
| 4 | 43% | 57% | 33% | ||||
| 5 and more | 10% | 11% | 10% | ||||
| Average charge | 3.6 | 3.8 | 3.5 | ||||
| Average length | 16.2 | 15.3 | 17 | ||||
| Sequence with internal E |
38% | 33% | 42% | ||||
| Fragmentation patterns |
N/A | Moderate cleavage for selected residue pairs |
Very strong cleavage at X-E |
||||
