Figure 3.

In vivo effect of injection of the α-gal glycolipids into B16 melanoma. Lesions reaching subcutaneous size of ~5 mm received one injection of α-gal glycolipids and were resected at different time points for histological analysis. (A) On day 2 post injection, mononuclear cells migrating from blood vessels (BV) are detected already; (B) On day 7 post injection, the infiltration is more extensive as indicated by the number of mononuclear cells migrating from blood vessels into the tumor; and (C) On day 7 post PBS injection, control tumors displayed no infiltration of mononuclear cells (×200).