Figure 7. An endogenous Tea1-For3 fusion protein is functional but impinges on the cell division machinery.

(A) Schematic of Tea1, For3, and Tea1-For3 protein domains and organization. (B) Anti-V5 immunoprecipitates from asynchronous tea1-V5₃, for3-V5₃, and tea1-for3-V5₃ cells were blotted with anti-V5 antibodies. Arrows indicate full-length proteins. Lysates were blotted with anti-Cdc2 as a loading control. (C) Fixed-cell DAPI/methyl blue images of stained wild-type, tea1Δ, for3Δ, tea1Δ for3Δ, and tea1-for3 cells. (D) Quantification of phenotypes of cells in (C), with three trials per genotype and n>300 for each trial. Data are presented as mean ± SEM for each category. (E) Quantification of cell lengths at cell division, with n>200 for each genotype. Data are presented as box-and-whisker plots showing the median (line in the box), 25^th–75^th percentiles (box), and 5^th–95^th percentiles (whiskers) for each genotype (Bar = 5 µm).