Table 1. Rates of ura4 loss (including genomic deletion, translocation, and mutation events), calculated using the method of the median.
| strainsa | Rate of ura4 lossb | Fold induction by Rtf1 expression (−/+ thiamine)c | Fold induction by the RFB (relative to the t-ura4-ori construct) | |
| + thiamine (Rtf1 repressed) | − thiamine (Rtf1 expressed) | |||
| t-ura4-ori | 4.6±1 | 4.3±1.8 | 0.9 | |
| t-ura4<ori | 4.1±0.9 | 13.9±2.8 | 3.4 (p = 0.002) | 3.2 |
| t-ura4<ori RTS1-d | 4.9±1.4 | 11.4±4.1 | 2.3 (p = 0.03) | 2.6 |
| t<ura4-ori | 3.9±1.3 | 4.8±2.5 | 1.2 | 1.1 |
| t>ura4<ori | 5.8±2.1 | 97.4±51.5 | 16.8 (p = 0.004) | 22.6 |
| t>ura4<ori RTS1-d | 4.9±2.2 | 19.5±3.3 | 4 (p = 0.006) | 4.5 |
| t>ura4-ori | 3.2±1.7 | 3.6±1.8 | 1.1 | 0.8 |
| t<ura4>ori | 6.6±1.7 | 50.3±14.2 | 7.6 (p = 0.014) | 11.7 |
a
the following nomenclature is used: “t” and “ori” refer to the telomere and the replication origin 3006/7, respectively; “<“ and ”>” indicate the RTS1-RFB and its polarity (< blocks replication forks moving from the ori3006/7 towards the telomere, and > blocks replication forks moving from the telomere towards the origin 3006/7).
b
event/cell/division ×10−8 ± standard error. Values are means of at least 3 independent rates.
c
statistical significance was determined using the nonparametric Mann-Whitney U test.