Skip to main content
. 2012 Oct 18;8(10):e1002965. doi: 10.1371/journal.ppat.1002965

Figure 1. Expression of LL3 in response to Plasmodium infection.

Figure 1

(A) An. gambiae mosquitoes were fed on mice infected with the non-gametocyte producing P. berghei ANKA 2.33 strain, with the non-invasive P. berghei MAOP mutant, or with the wild type P. berghei ANKA 2.34 strain (WT). Midguts were dissected from sugar-fed (0 h) mosquitoes or at different time points following blood meal. LL3 and SRPN6 mRNA abundance was measured by qRT-PCR and normalized to rpS7 (relative gene expression). Data from three independent biological experiments were pooled and analyzed by two-way ANOVA and asterisks denote significant differences (P<0.05) when compared to sugar-fed samples using a Dunnet's post-test. (B) LL3 and SPRN6 mRNA abundance was measured by qRT-PCR in midguts from mosquitoes that were sugar-fed (0 h), at different times after a P. falciparum-infected blood meal or at 24 h after a non-infectious blood meal (Non-inf). Data from two independent biological experiments were pooled and analyzed by a one-way ANOVA. Asterisks denote significant differences (P<0.05) when compared to sugar-fed samples using a Dunnet's post-test.