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. 2012 Oct 18;8(10):e1002975. doi: 10.1371/journal.ppat.1002975

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© 2012 Ishibashi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Protoplasts isolated from transgenic BY-2 cells expressing tm-1^GCR26, Tm-1^GCR237, or Tm-1^I91T, or non-transgenic BY-2 cells were inoculated with TLIle, ToMV-L, LT1, T21, LT1^E979K, or LT1^D1097Y by electroporation. At 20 hpi, protoplasts were harvested and coat protein (CP) accumulation was analyzed by SDS-PAGE and Coomassie blue staining.