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. 2012 Oct 18;7(10):e47257. doi: 10.1371/journal.pone.0047257

Figure 4. Hair cell regeneration is blocked by inhibition of mitosis.

Figure 4

ET4:GFP larvae were treated at 5 dpf with neomycin or copper for 1 h and incubated in flubendazole for 48 h. (A) Those not treated with flubendazole continued to add mature hair cells. Two-way ANOVA found significantly more cells expressed GFP than parvalbumin (p<0.001), indicating the presence of hair cell precursors. Bonferroni post-hoc analysis confirmed a significant difference in control and ototoxin-treated groups between GFP- and parvalbumin-positive hair cell counts at 48 hpt (**, p<0.01; ***, p<0.001). (B) Those treated with flubendazole exhibited little or no increase in mature hair cells. There were at most 1 or 2 parvalbumin-negative precursors present per neuromast, indicating that undifferentiated precursors did not accumulate. N = 8 fish per group. Error bars are +/− SD.