Figure 6. Regeneration is obscured by growth of the immature lateral line.

(A, B) Wild type embryos were treated at 3 dpf with serial dilutions of neomycin or copper for 30 min. Hair cell regeneration was scored using FM 1-43FX and normalized to controls. Complete regeneration was observed in all groups. N = 14 fish per group. (C) Individual counts of hair cells labeled with antibodies against parvalbumin confirmed complete regeneration after 1 h treatment with copper or neomycin. However, all ototoxin-treated groups closely matched control embryos, which had few hair cells at the time of treatment. Instead of regeneration, most hair cell addition appeared related to early development of the lateral line. N = 8 fish per group. Error bars are +/− SD.