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. 2012 Oct 18;7(10):e47468. doi: 10.1371/journal.pone.0047468

Figure 4. ATP, ADP and AMP hydrolysis by human MB cell lines.

Figure 4

Confluent cultures of Daoy, ONS76 and D283 cells were incubated with ATP or ADP or AMP as described in the Materials and Methods section. For D283 cells, a concentration of 2.0 mM and an incubation time of 30 minutes were used for all substrates, whereas for Daoy and ONS76 cell lines, 1.0 mM and 30 minutes of incubation to ATP and ADP, and 2.0 mM and 10 minutes of incubation to AMP were used. Specific activities were expressed as nmol Pi/min/mg of protein. Bars represent mean ± SD of four independent experiments performed in triplicate. Data were compared by Two-Way ANOVA test following by Bonferroni post hoc test. (***) p<0.001 and (*) p<0.05 compared to control data.