Figure 5. Metabolism of extracellular AMP by MB cell lines.

Confluent cultures of Daoy (A), ONS76 (B) and D283 cells (C) were cultivated in 24 well plates until confluence and incubated with 100 µM AMP in 250 µL of incubation medium as described in Materials and Methods. Aliquots of the supernatant were collected at 0, 5, 10, 20, 30, 60 and 90 min, and levels of AMP, ADO, INO and HIPO (hipoxantine) were determined by HPLC. Data were expressed as mean ± SD values from two independent experiments performed in triplicate.