Skip to main content
. 2012 Oct 18;7(10):e47468. doi: 10.1371/journal.pone.0047468

Figure 8. Flow cytometry analysis of ecto-5′-NT/CD73 protein expression in Daoy, ONS76 and D283 MB cell lines.

Figure 8

Confluent MB cell lines were trypsinized and prepared as described in Materials and Methods. After incubation with purified mouse anti-human CD73 antibody (1∶10) and labeling with Alexa fluor 555 Rabbit anti-mouse IgG (1∶100), cells were analyzed by flow cytometry. (A) Results are shown as the ratio of labeled over non-labeled cells. (B) Representative graphic of ecto-5′NT/CD73-positive cells. Statistical relevance was analyzed by the One-Way ANOVA test following by the Tukey post hoc test. The experiments were performed in triplicate. (**) p<0.001 compared to control data.