Table 1. Ectonucleotidases primer sequences for PCR experiments.

	Primer sequence	(T°C)	Fragment size (bp)
NPP1 F	5′- TGG GTT GAA ACC AAG CTG TGC CA -3′	69	94
NPP1 R	5′- ACA GGC AGC ATC ACA GCG ACA -3′
NPP2 F	5′- AGG AGG AGC TCG TTC CAG T -3′	59	116
NPP2 R	5′- TCC CAT CCT TCT GCT CTC TT -3′
NPP3 F	5′- GCA GGT GGA CCA GTC AGT GCC -3′	69	90
NPP3 R	5′- CCG CTG CTT CAG GCC TTC CA -3′
NTPDase1 F	5′- AGT ATG GGA TTG TGC TGG ATG -3′	54	147
NTPDase1 R	5′- TCT GAA CAA ATT TTG AGA TTC CAG -3′
NTPDase2 F	5′- CAG GAT GTG CCC AAA GAG A -3′	61	685
NTPDase2 R	5′- CCC CAT TGA AAG AGC ATC G -3′
NTPDase3 F	5′- TTT CCC TGG ACA CCT TCA AC -3′	55	184
NTPDase3 R	5′- TGT ATT TGG GGC CAA GTC TC -3′
NTPDase5 F	5′- GCA TTT GCC AAC ACC TTT TT -3′	55	179
NTPDase5 R	5′- ACA GGG CTC TCT GTG ATG CT -3′
Ecto-5′NT/CD73 F	5′- AGG GGT GTG GAC GTC GTG GT -3′	65	84
Ecto-5′NT/CD73 R	5′- CCC AGC AGG CAC CTC TTT GGA -3′
ADA F	5′- GGG GAG CGA GAC TTC CGG GGT -3′	69	83
ADA R	5′- TCC ACC ACC TTG GGG GAC CA -3′
ALP F	5′- CAT TGG CAC CTG CCT TAC TA -3′	59	104
ALP R	5′- GCT CCA GGG CAT ATT TCA GT -3′
GAPDH F	5′- TTC TTT TGC GTC GCC AGC CG -3′	64	93
GAPDH R	5′- ACC AGG CGC CCA ATA CGA CC -3′

Primer sequences for PCR experiments. These primers listed here were used for both RT-PCR and real time PCR reactions with exception of primers for amplification of NTPDase2 coding sequences. Melting curve analysis was performed to determine the specificity for each real-time PCR reaction.