Skip to main content
. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Methods Mol Biol. 2012;832:65–80. doi: 10.1007/978-1-61779-474-2_3

Figure 2.

Figure 2

The strategy for analyzing neuron-specific protein ubiquitination in the fly. The neuronal elavGAL4 driver allows the expression of BirA (negative control) or Ub6-BirA in neurons using the GAL4/UAS system. Biotinylated Ub-conjugates are isolated and subjected to 1D SDS gel analysis followed by in-gel digestion and LC-MS/MS.