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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Shock. 2012 Nov;38(5):515–523. doi: 10.1097/SHK.0b013e31826f862c

Fig. 4. Skewed CD4 T cells from post septic mice produce increased levels of IL-17.

Fig. 4

CD4 T cells were MACS enriched from the spleens of sham treated or post septic (CLP) mice on day 14 and assayed for cytokine production in vitro. (A) Cells were re-stimulated with anti CD3 and anti CD28 in the presence of golgi-plug overnight in vitro and stained intracellularly for IL-17 levels and analyzed by flow cytometry. (B) Cells were seeded into 96 well plates at a density of 1 × 106 cells per well. The cells were either un-skewed (Th0) or skewed towards Th1, Th2 and Th17 for 48 hours. The supernatants were then collected and analyzed for Th1, Th2 and Th17 cytokines by bead-based multiple cytokine analysis (Luminex).