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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Immunol Cell Biol. 2012 Apr 24;90(9):912–915. doi: 10.1038/icb.2012.21

Figure 2. ICAM-1 blockade significantly inhibits Toxoplasma gondii tachyzoite migration across simulated human retinal, but not choroidal, vascular endothelium.

Figure 2

Graphs showing number of tachyzoites recovered from lower chambers of transwells divided by (A and B) human retinal endothelial cell monolayers or (C) human choroidal endothelial cell monolayerscultured on type 1 collagen, 4 hours after upper chambers were loaded with 1 × 106 live or heat-killed RH strain T. gondii tachyzoites (n=6 wells, parasite viability = A, 55%; B, 52%;C, 95%). Monolayers were pre-incubated for 2 hours with (A and C) mouseanti-human ICAM-1 IgG1 (α-ICAM-1) or (B) mouse anti-human VCAM-1 IgG1 (α-VCAM-1), or control mouse IgG1 (control). There was a significant reduction in tachyzoite migration across the retinal endothelial monolayer in the presence of anti-ICAM-1 antibody (***p < 0.001), but not anti-VCAM-1 antibody (p > 0.05) (Student’s t-test). Anti-ICAM-1 antibody did not reduce tachyzoite migration across the choroidal endothelial monolayer (p > 0.05) (Student’s t-test). Each graph presents results that are representative of two independent experiments. In all graphs, bars represent mean and error bars represent standard error of mean.