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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Cancer Res. 2012 Aug 9;10(10):1389–1399. doi: 10.1158/1541-7786.MCR-12-0136-T

Figure 2. Molecular and biological characterization of IMECs with different Smad3 levels.

Figure 2

A. Western blot showing relative levels of total and C-terminally phosphorylated Smad2 and Smad3 in IMECs of different Smad3 (S3) genotypes. B. Electrophoretogram (left) and corresponding “virtual blot” image (right) of SimpleWestern analyses of IMEC lysates simultaneously probed for Smad3 and alpha-tubulin as an internal loading control. Smad3/tubulin ratios normalized to the Smad3 wildtype condition are shown below the virtual blot image. C. Phase contrast images of IMEC morphology in monolayer culture. D. Growth characteristics of the IMEC cultures, assessed by quantitative imaging of culture confluence in real time using an IncuCyte Imaging system. Results are mean +/- SEM (n=3).