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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Mol Cancer Res. 2012 Aug 9;10(10):1389–1399. doi: 10.1158/1541-7786.MCR-12-0136-T

Figure 3. Biological responses to TGF-β in IMECs with different Smad3 levels.

Figure 3

A,B. Effect of Smad3 genotype on growth inhibitory (A) or apoptotic (B) responses of IMECs to TGF-β. Results are mean +/- SEM for 3 determinations and are normalized to the untreated control for each genotype. * p<0.05, two-way ANOVA. C. The lack of an apoptotic response in Smad3+/- cells was confirmed in three independent isolates. Results are mean +/- SEM for 3 determinations and are presented without normalization to show similarity in basal apoptosis levels between genotype groups. D. Conventional Western (left) and SimpleWestern “virtual blot” (right) showing quantitative restoration of Smad3 to wildtype levels in Smad3+/- cells following lentiviral-mediated addback of Smad3. CON is a control lentivirus with stuffer sequence in place of Smad3. E. Restoration of apoptotic response to TGF-β on add-back of Smad3 to Smad3+/- IMECs. Results are expressed as in C. F,G. Effect of Smad3 genotype on migration (F) and invasion (G) responses to TGF-β. Results are mean +/- SEM for a representative experiment (n=10-20 fields /condition) normalized to the untreated Smad3+/+ condition. * p<0.05, two-way ANOVA with Bonferroni correction.