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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Psychiatry. 2012 Jul 17;18(5):557–567. doi: 10.1038/mp.2012.97

Figure 3. SR level is decreased in primary astrocytes and brain tissue from the GFAP-ΔC hDISC1 mice.

Figure 3

[a] Both DISC1 and SR were expressed in astrocytes in 6-week-old mouse brain. DISC1 and SR immunofluorescence was carried out with glial marker GFAP in the mouse forebrain sections, scale bar - 20 µm;

[b] ΔC-hDISC1 is expressed in the astrocytes of the mutant mouse but not in the neurons or in the control mouse;

[c] Immunofluorescence showing that mutant DISC1 is expressed in astrocytes of the mouse hippocampus, scale bar - 10 µm;

[d] Expression time course of ΔC-hDISC1 in the mutant mouse forebrain, with the highest expression level around E18. PC, positive control, brain tissue from a mouse that expresses mutant DISC1 under the CAMKII promoter;

[e] Expression of SR, ΔC-hDISC1, endogenous mouse DISC1 and an astrocytic marker, ALDH1L1, in samples from primary astrocytes. Below, quantitative evaluation of expression of these markers, n=7–10 per group, *p<0.05, ** p<0.005 vs. controls, Student’s t-test. The exposure time of the ΔC-hDISC1 and endogenous mouse DISC1 blots is the same.

[f] Expression of SR, ΔC-hDISC1, endogenous mouse DISC1 and an astrocytic marker, ALDH1L1, in samples from forebrain area of newborn mice. Below, quantitative evaluation of expression of these markers, n=7–9 per group, *p<0.05 vs. controls, Student’s t-test.

[g] D-serine level in the culture media of primary astrocytes. L-serine was added to the culture media to a final concentration of 4 mM and D-serine level was measured 48 h later. Control, n=6; mutant, n=7, *** p<0.0005;

[h] D-serine level (expressed as [D-serine]/[total serine]) in the brain of P0, P7, P21 and P90 mice determined by HPLC. Each bar represents ~4–6 mice. In the P0 mouse cortex, mutant has ~20% decrease in D-serine level compared to the control, while in the P21 mouse cortex and hippocampus, there is a small but significant increase, *p<0.05, ** p<0.005, NS, not significant. CTX - cortex.

[i] SR mRNA level in primary astrocytes is unaltered as measured by quantitative Real Time-PCR. Control, n=7; mutant, n=6.