Scheme 1.

Synthesis of B-ACV. Biotin-Acyclovir (B-ACV): White solid; LC/MS (m/z): 452.1; ¹HNMR(DMSO-d6): δ 1.24–1.35 (m, 2H), 1.39–1.51 (m, ³H), 1.54–1.63 (m, 1H), 2.17–2.24 (m, 2H), 2.55–2.58 (m, 1H), 2.79–2.84 (m, 1H), 3.05–3.09 (m, 1H), 3.64–3.67 (m, 2H), 4.07–4.15 (m, ³H), 4.29–4.32 (m, 1H), 5.34 (s, 2H), 6.37 (brs, 1H), 6.43 (brs, 1H), 6.55 (brs, 1H), 7.81 (s, 1H), 10.69 (brs, 1H); ¹³C NMR(DMSO-d6): 24.44, 27.96, 33.17, 55.38, 59.20, 61.06, 62.63, 66.59, 71.83, 116.48, 137.74, 151.45, 153.95, 156.83, 162.76, 172.79