Table II.
Stability of 23mer and 24mer in Rat Plasma
| Freeze–thaw stability(−20°C) (3 cycles) | Room temp. stability(8 h) | Autosampler stability(24 h, 5°C) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nominal conc. (ng/mL) | 30 | 150 | 30 | 150 | 30 | 150 | ||||||
| 23mer | 24mer | 23mer | 24mer | 23mer | 24mer | 23mer | 24mer | 23mer | 24mer | 23mer | 24mer | |
| Obsd conc. (ng/mL) | 31.5 ± 1.8 | 31.2 ± 2.7 | 161 ± 9 | 136 ± 6 | 31.3 ± 2.0 | 28.5 ± 2.1 | 156 ± 16 | 134 ± 4 | 32.6 ± 2.7 | 29.7 ± 1.2 | 153 ± 12 | 140 ± 15 |
| Accuracy (%) | 105 | 104 | 107 | 90.4 | 104.2 | 95.1 | 104 | 89.4 | 109 | 99.0 | 102.2 | 93.1 |
| RSD (%) | 5.81 | 8.76 | 5.82 | 4.09 | 6.31 | 7.28 | 10.2 | 2.82 | 8.18 | 4.04 | 7.63 | 10.4 |