Fig. 1.

The left panel shows the typical lipolysis set up. The thermo-jacketed reaction vessel (I) contains the digestion medium and is stirred constantly by the magnetic stirrer (II). The pH-electrode (III) measures the pH and temperature of the lipolysis medium. The pH-stat unit (IV) regulates the dispensing of sodium chloride (V) and calcium chloride (VI). The water bath (VII) maintains the temperature of the lipolysis medium at 37°C. The right panel depicts the typical profile of a lipolysis experiment where the pH of the lipolysis medium is maintained constant (blue line). The red line represents the cumulative consumption of NaOH to titrate the liberated fatty acids during in vitro lipolysis