Table II.
Examples of In Vitro Lipolysis Conditions
| Volume (ml) | Duration (min) | pH | Buffer | Lipase | Bile/phospholipid | Calcium | Substrate | References |
|---|---|---|---|---|---|---|---|---|
| 9 | 12 | 6.5 | Tris-maleate (2 mM) | Pancreatin (24 TBU/ml) | NaTDC (8 mM) | 10 mM | 0.45 ml TG emulsion | (50) |
| 5 | 8.5 | Tris-maleate (50 mM) | Porcine pancreas (168–280 TBU/ml) | NaTDC (0–30 mM/lecithin) | 0–30 mM | 0–5% (w/v) of TG emulsion (10%) emulsified with PL | (34) | |
| 10 | 6–9 | Tris-maleate (2 mM) | Purified from human pancreatic fluid (1–8 TBU/ml) | NaTC NaTDC or NaCDC (6 mM) | 10 mM | 0.5 ml olive oil emulsified with gum Arabic | (24) | |
| 40 | 30 | 6.8–7.4 | Tris-maleate (50 mM) | Porcine pancreatin ((8× USP) 1,000 IU/ml) | NaTC (5 mM l-α-PC) | 5 mM | 0.2–1 g TG | (51,52) |
| 37.5 | 20–120 | 7.0 | Phosphate (5 mM) | Porcine pancreas type II (L3126, Sigma; (100–400 U/mg, 2.4 mg/ml) | Porcine bile extract (20 mM) | 10 mM | 0.5% TG emulsion,different emulsifiers,1% | (53) |
| 300 | 40–90 | 6.5 | Tris-maleate (2 mM) | Porcine pancreatin (3× USP; 300–800 USP units/ml) | Porcine bile extract (5–30 mM)/1–5 mM PC | Continuous addition (0.045–0.181 mmol/min) | 15 mM -30 mM TG,1-3 g SNEDDS, | (37,48,54,55,56) |
| 10–40 | 30–60 | 7.5 | Tris-maleate (50 mM) | Porcine pancreatin (8× USP; 1,000 TBU/ml) | NaTDC (5–20 mM)/1.25–5 mM lecithin (60% PC) | 5 mM | 1 g SEDDS , TG | (57,58) |
| 100 | 40 | 6.5 | Tris-maleate (50 mM) | Pancreatin extract (8 TBU/mg) | Bile salts (5 mM)/1.25 mM lecithin | 5 mM | 1 g SEDDS | (59) |
| 20 | 35 | 7.5 | Tris-maleate 50 mM | Porcine pancreatic lipase 40,000 IU/g 4,000 TBU/ml | NaTDC 5 mM/1.25 mM lecithin (92% PC) | 5 mM | 0.21 g TG, sub-microemulsion | (60) |
FFA free fatty acids, NaTC sodium taurocholate, NaTDC sodium deoxytaurocholate, NaCDC sodium chenodeoxycholate, PC phosphatidylcholine, PL phospholipid, TBU Tributyrin unit (1 TBU equals the amount of enzyme that can liberate 1 mol of fatty acid from tributyrin/min) (69)