Table 1.
Recent studies using transcriptomics and proteomics methods to study the action of androgen in teleost fishes
| Species | Sex and stage | Tissue | Androgenic treatment | Concentration | Duration (days) | Biological material | Gene ontology (prominent examples) | References |
|---|---|---|---|---|---|---|---|---|
| Atlantic cod (Gadus morhua) | Adult female | Oocytes (pre-vtg) | T and 11-KT | 10 and 100 μM | 5 and 10 | Transcripts | Stress (HSPs), reproduction (steroid metabolism), cell cycle (cyclin) | Kortner et al. (2008) |
| Common carp (Cyprinus carpio) | Juveniles | Liver | 17α-MT, 11-KT | 0.1, 1.0, 10 μg MT/L and 5, 50, 500 ng 11-KT/L and 5 μg MT or 11-KT/g i.p. | 1 and 4 | Transcripts | Immune response, lipid transport, oogenesis, protein metabolism, proteolysis | Moens et al. (2007) |
| Fathead minnow (Pimephales promelas) | Adult female | Liver | TB | 5.0 μg TB/L | 2 | Proteins | Mutagenesis, apoptosis, cell cycle regulation, catabolism | Martyniuk et al. (2009) |
| Fathead minnow (Pimephales promelas) | Adult female | Liver | TB | 5.0 μg TB/L | 2 | Proteins | Xenobiotic clearance, LDL oxidation, smooth muscle cell proliferation (Table 2) | Martyniuk and Denslow (this review) |
| Fathead minnow (Pimephales promelas) | Adult female | Ovary | TB | 0.5 μg TB/L | 2 | Transcripts | Secretion, embryonic development, immune response, development, regulation of apoptosis, system development, proteolysis, muscle development | Garcia-Reyero et al. (2009)a |
| Fathead minnow (Pimephales promelas) | Adult female | Ovary | TB | 1.0 μg TB/L | 4 | Transcripts | Protein ubiquitination, protein biosynthesis, lipid metabolism, transcription, DNA repair, and DNA replication | Dorts et al. (2009)b |
| Qurt medaka (Oryzias latipes) | Larvae (both) | Whole tissue | 11-KT | 100 μg 11-KT/L | 7 | Transcripts | Metabolism, stress responses, iron storage, mitotic regulation, neural networks, reproductive physiology, sex differentiation and growth | Leon et al. (2008) |
| Rainbow trout (Oncorhynchus mykiss) | Adult female | Liver | TB | 1.0 μg TB/L | 7 | Transcripts | Protein binding, transport, ATP regulation, lipid binding, copper and calcium ion binding, ribosomes, immune, cell cycle, | Hook et al. (2006)c |
| Zebrafish (Danio rerio) | Adult female | Liver | MDHT | 0.1, 0.7, 4.9 μg MDHT/L | 1 and 7 | Transcripts | Steroid metabolism, hormone transport, regulation of cell growth and proliferation polyamine biosynthesis | Hoffmann et al. (2008) |
The table includes categories of gene ontology that were prevalent in each study (i.e., major themes reported).
aBiological processes shown in this table are those from Garcia-Reyero et al. (2009) that were represented by >100 genes on the FHM microarray (P < 0.03).
bBiological processes shown in this table are those from Dorts et al. (2009) that had >10 genes in the gene ontology category.
cMajor biological themes identified by Hook et al. (2006) that contained >2% genes differentially expressed compared to all regulated genes.
i.p., intraperitoneal injection.