Figure 5.

Gabapentin prevents the development of muting competence. A, Representative DIV 10–12 autaptic EPSCs after 4 h control treatment (30 mm NaCl) or depolarized (30 mm KCl). Astrocyte-rich cultures were treated with 32 μm gabapentin at DIV 7 (1× GBP). B, Representative DIV 10–12 autaptic EPSCs after 4 h control or depolarized. Astrocyte-rich cultures were treated with 32 μm gabapentin at DIV 7 followed by half-media switches of fresh 32 μm gabapentin in astrocyte-conditioned medium every day (3–5× GBP). Summary data for A and B are given in the Results. C, Spontaneous activity in DIV 11–13 mass cultures after 4 h control treatment (30 mm NaCl) or depolarized (30 mm KCl). Cultures were treated at DIV 7 with or without 32 μm gabapentin followed by daily half media switches with astrocyte-conditioned medium (CM) or CM plus 32 μm gabapentin (3–5× GBP). EPSCs and IPSCs were measured simultaneously at −35 mV. D, Summary of total spontaneous synaptic activity (EPSC + IPSC points) in DIV 11–13 mass cultures, normalized to control (CM, NaCl) for each recording day (n = 23 neurons; *p < 0.05, Bonferroni corrected).