Skip to main content
. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: Bioconjug Chem. 1995 May-Jun;6(3):255–263. doi: 10.1021/bc00033a004

Table 4.

Relationship between Concentration of Compound 19 and the Ability of Hydroxylamine to Restore Binding of [3H]CPX

concn of
19 (nm)		 inhibition of [3H]CPX
binding (% control)
 reversal of
inhibition
(% control)
(−H2NOH) (+H2NOH)
100 54.4 34.5 20
250 63.3 ± 1.2 43.6 ± 4.9 20
500 76.0 ± 3.0 54.0 ± 4.0 22
a

Data are means ± s.e.m. of three experiments or a single experiment. After incubation with the isothiocyanate derivative at 37 °C for 1 h, membranes were washed three times, incubated with hydroxylamine (500 mM, 37 °C for 1 h), washed twice, and then incubated with 0.2 nM [3H]CPX (14). When the hydroxylamine incubation was carried out at 25 °C for 1 h, the degree of reversal was 8% (100 nM 19), 9% (250 nM 19), or 15% (500 nM 19).