Table 5.
Relationship between Temperature and the Regeneration by Hydroxylamine of [3H]CPX Binding Following Inhibition by Compound 19
| reversal of inhibition (% control) |
||
|---|---|---|
| concn of 19 (mM) | 37 °C | rt |
| 250 | 24 ± 3 | 9 ± 5 |
| 500 | 23 ± 2 | 15, 15 (n = 2) |
a
Data are means ± s.e.m. of three experiments, unless noted. After incubation with the isothiocyanate derivative 19 at 37 °C for 1 h, membranes were washed three times, incubated with hydroxylamine (500 mM) at 37 °C for 1 h, washed three times, and then incubated with 0.2 nM [3H]CPX (14). Regeneration following overnight incubation with hydroxylamine at room temperature resulted in regeneration comparable to 1 h at 37 °C (data not shown).