Figure 2.
2H-NMR spectra of 2H-labeled samples of the Glc derivative 1,2-O-isopropylidene-d-glucofuranose. Top trace, Glc was synthesized from Fru-6-P via phosphoglucoisomerase and Glc-6-phosphatase in 2H-labeled water. Middle trace, Glc was synthesized in 2H-labeled water from dihydroxyacetone phosphate via triose phosphate isomerase, aldolase, Fru bisphosphatase, phosphoglucoisomerase, and Glc 6-phosphatase. Bottom trace, Intact bean leaves were placed at the beginning of darkness in plastic bags containing paper soaked with 2H-labeled water, the leaves were harvested after 3 h and treated with invertase, and the Glc liberated from Suc was derivatized.