Figure 1. Expression of gp120 protein in transduced DC2.4_gp120 and EL4_gp120 clones.

(A and B) Cytosolic proteins were isolated from transduced DC2.4_gp120 clones (C1) and C4 (A) and EL4_gp120 clones C135 and C149 (B). Each sample (20 μg) was assessed by Western blotting for gp120 expression. Protein extract from parental DC2.4 or EL4 was included as a negative control, and purified gp120 protein (500 ng) was included as a positive control. Data are representative of at least three separate experiments.