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. 2012 Aug 30;287(43):35796–35803. doi: 10.1074/jbc.M112.379156

FIGURE 5.

FIGURE 5.

Thermo-induced conformational changes and proteolysis of RovA mutant proteins. The stability and thermo-induced conformational changes of the RovA mutants RovA(G116A) (A) and RovA(S127I/G128K) (C) were investigated at 37 °C. A higher molecular weight protein (C) that reacted with the polyclonal antisera was used as a loading control. Thermo-induced conformational changes in RovA(G116A) (B) and RovA(S127I/G128K) (D) were detected by CD spectroscopy. CS spectra, degrees (m−1 cm−1) versus wavelength of RovA(G116A) (0.2 mg/ml) or RovA(S127I/G128K) (0.2 mg/ml) at 20 and 37 °C, are presented.