FIGURE 1.

Domain structure and conserved features of CTRP12. A, four domains of CTRP12 protein: a signal peptide (SP); an N-terminal domain with the highly conserved N-glycosylation site (Asn³⁹), Cys⁸⁵, and polybasic cleavage motif KKXR; a collagen domain with six Gly-X-Y repeats; and a globular C1q/TNF-like domain with three conserved Cys residues (Cys¹⁶², Cys²⁴¹, and Cys²⁴⁶) and two potential N-glycosylation sites (Asn²⁸⁷ and Asn²⁹⁷). Endopeptidase cleavage at Lys⁹¹ (indicated by arrow) generates the gCTRP12 isoform. B, sequence alignment of mouse, human, chicken, Xenopus, and zebra fish CTRP12. Highlighted are the conserved Asn³⁹ shown to be glycosylated in this study, the cleavage motif KKXR, and the Cys⁸⁵ shown in this study to be important for disulfide-linked multimerization of CTRP12.