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. 2012 Aug 31;287(43):35804–35814. doi: 10.1074/jbc.M112.365965

FIGURE 2.

FIGURE 2.

CTRP12 is a secreted multimeric glycoprotein. A, immunoblot analysis of CTRP12 expressed in HEK 293T cells. Empty vector (pcDNA3.1) was used as negative control. P, pellet; S, supernatant. B, Coomassie Blue staining of purified recombinant CTRP12 on a SDS-PAGE gel. C, CTRP12 treated with (+) or without (−) PNGase F. D, immunoblot analysis of wild-type (WT) CTRP12 and its N39A mutant. E, immunoblot analysis of gCTRP12 treated with (+) or without (−) PNGase F (left) and the gCTRP12 mutants. F, immunoblot analysis of WT CTRP12 and its C85A mutant treated with (+) or without (−) β-mercaptoethanol (β-ME). Arrows indicate the monomeric, trimeric, and higher order molecular mass oligomeric form of CTRP12.