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CTRP12 mutants with enhanced or suppressed cleavage. A, immunoblot analysis of secreted WT CTRP12 and its R93A mutant. B, immunoblot analysis of secreted WT CTRP12 and its triple mutant (K90A/K91A/R93A; designated as 3M). Media containing the recombinant proteins from transfected HEK 293T cells were collected 48 h after transfection and subjected to immunoblot analysis.
