FIGURE 7.
fCTRP12 and gCTRP12 preferentially activate Akt or MAPK signaling in 3T3-L1 adipocytes. A, immunoblot analysis of Akt (Thr308) and p44/42 MAPK (Thr202/Tyr204) phosphorylation in 3T3-L1 adipocytes stimulated with vehicle control or 10 μg/ml recombinant CTRP12 for 15 min. Blots were also probed for total IRS1 and Akt protein. B and C, quantification of Akt (B) and p44/42 MAPK (C) phosphorylation as shown in A, normalized to their respective total protein and then normalized to value obtained from cells treated with vehicle control. *, p < 0.05; **, p < 0.01; #, p < 0.001 (n = 3). Error bars, S.E.