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. 2012 Aug 31;287(43):35815–35824. doi: 10.1074/jbc.M112.380493

FIGURE 6.

FIGURE 6.

Endosomal localization enhances SARA down-regulation by PI3K inhibition. A, serum-deprived HKC were preincubated in isotonic media either containing potassium (K+) or without (K−) for 30 min prior to addition of either vehicle control (V) or 20 μm LY294002 (LY) for 6 h. Significant variation from control values are only seen under K+ conditions, where LY294002 reduces SARA expression (*, p = 0.0106) and enhances αSMA (*, p = 0.0001). B, HKC were transfected with either FLAG-tagged WT-SARA or FLAG-tagged ΔFYVE-SARA for 3 h followed by treatment with either control vehicle (V) or 20 μm LY294002 (LY) for an additional 16 h. β-Actin is shown as a loading control. All levels of SARA are significantly lower than untreated WT SARA expression (*, for WT+LY294002, p < 0.0001; ΔFYVE+vehicle, p = 0.0006; ΔFYVE+LY294002, p = 0.0004). However, there is no further decrease by LY294002 in ΔFYVE-expressing cells (p = 0.3290).