FIGURE 9.

4-HNE specifically enhances the expression of the PPARγ target gene Lpl in cultured motor neuron-like cells. Expression of PPAR isoform-specific target genes, i.e. Lpl for PPARγ (A), Mcad for PPARα (B), and Acsl6 for PPARβ/δ (C), was analyzed in NSC-34 cells treated with the vehicle (veh), 4-HNE (40 or 60 μm), or pioglitazone (30 μm) for 24 h (n = 3 in triplicate). Data are expressed as mean ± S.E. *, p < 0.05 versus vehicle; **, p < 0.01 versus vehicle, one-way ANOVA followed by Newman-Keuls post hoc test.