FIGURE 3.

L-selectin-tethered lubricin is shed off upon activation. The levels of L-selectin and lubricin on peripheral PMN from healthy controls were monitored by flow cytometry. A, level of L-selectin on PMN before (blue line) and after (red line) stimulation with TNF. B, level of lubricin on PMN before (blue line) and after (red line) stimulation with TNF. C, the association between resting PMN and lubricin in the absence (blue line) and presence (red line) of exogenous lubricin purified from SF. Neg. Ctrl., negative control.