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. 2012 Aug 28;287(43):35922–35933. doi: 10.1074/jbc.M112.363119

FIGURE 5.

FIGURE 5.

Sialylated and sulfated core 2 O-glycans on SF lubricin determined by LC-MS in the negative ion mode. A, MS/MS spectrum of a desialylated core 2 oligosaccharide with a [M − H]− ion of m/z 749. The inset shows the expanded region of linkage-specific 0,2A ions for type 2 (Galβ1–4GlcNAc) linkage. B, MS/MS spectrum of a desialylated sulfated core 2 oligosaccharide with an [M − H]− ion of m/z 829. The inset shows the MS3 spectrum of the fragment ion at m/z 282, which is consistent with GlcNAc6S; C, MS/MS spectrum of sialylated and sulfated core 2 O-glycan with an [M − 2H]2− ion of m/z 706. D, MS/MS spectrum of an sLex-containing core 2 O-glycan with an [M − 2H]2− ion of m/z 738. Proposed structures and fragments are featured as insets in each spectrum. The nomenclature of fragments is based on the rules introduced by Domon and Costello (68).