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. 2012 Sep 5;287(43):35975–35984. doi: 10.1074/jbc.M112.372458

FIGURE 2.

FIGURE 2.

TGFβ inhibits Ocn expression and stimulates vimentin expression post-transcriptionally. A, Northern blot analysis of total RNA from primary calvarial osteoblasts showing that TGFβ inhibits endogenous Ocn expression dose-dependently. B, Western blot analysis of total protein from primary calvarial osteoblasts. C, Northern blot analysis with indicated cDNA probes showing that TGFβ does not affect endogenous vimemtin mRNA level in calvarial osteoblasts. D, Western blot analysis showing that TGFβ stimulates vimenitn protein in calvarial osteoblasts. E, Western blot analyses showing that rhTGFβ1 (0.2 ng/ml) stimulates vimentin protein expression in the indicated primary osteoblasts and osteoblastic cell lines. F, Northern blot analyses showing that rhTGFβ1 (0.2 ng/ml) does not affect endogenous vimentin mRNA level in the indicated primary osteoblasts and osteoblastic cell lines.