The shift to vesicles for the Ser-340/341 mutant is also induced by negative charge and is selective.
A, phospho-mimetic mutant Flag-ATP7BR875,S340/341D is localized to vesicles in basal (upper panel) and copper-depleted (100 μm BCS for 2 h) conditions (lower panel). B, Flag-ATP7BR875,S478A, Flag-ATP7BR875,S481A, and Flag-ATP7BR875,S1453A behave as wt Flag-ATP7BR875. They are located in the ER in basal conditions, and traffic to vesicles upon treatment with 100 μm CuCl2 for 2 h (Scale bar: 5 μm).