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. 2012 Aug 16;287(43):36041–36050. doi: 10.1074/jbc.M112.370403

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — The Ser340/341A mutation reduces interactions between N-ATP7B and nucleotide-binding domain (NBD) and alters conformation of ATP7B. A, cell lysates containing maltose-binding fusions of N-ATP7B (WT) or N-ATP7B^S340/341A (S→A) were mixed with lysates containing recombinant NBD and passed over amylose resin. Purified NBD (upper panel) and N-ATP7B (middle panel) were detected using corresponding antibodies and proteins were quantified by densitometry (lower panel). D, top: proteolytic pattern of Flag-ATP7B^R875 (WT) or Flag-ATP7B^{R875,S340/341G} (S→G) following 15 min treatment with trypsin. Fragments were resolved using 8% SDS gel and immunoblotted with anti-NBD-ATP7B antibody. Bottom: bands intensity was compared by densitometry.